2024年5月27日 星期一
爱知结球白菜组培再生株与实生株的性状比较
Comparison of some characters between in vitro regenerated plants and seedling plants of Brassicacam pestris ssp. pekinensis ‘Aichi Hakusai’
2005年 第14卷 第1期 页码[31-34]    下载全文[0.6MB]  
摘要

对日本引进的爱知结球白菜〔Brassica campestris ssp. pekinensis‘Aichi Hakusai’〕的组培再生株从染色体数目、形态特征及生化特性方面与实生株进行了比较。结果表明,再生株染色体数目与实生株相同,均为 2n=20;再生株在移栽初期长势不齐, 1个月后,生长趋于一致,形态指标均显著高于相同条件下栽培的实生株;两者叶片可溶性糖和可溶性蛋白质含量的变化趋势相似;可溶性蛋白质含量在移栽初期高,碳氮比 (C/N)小,代谢类型主要是氮素代谢;随着生长发育的进行,叶面积增加,碳水化合物代谢加强,其可溶性糖含量增加,C/N比逐渐上升。说明爱知结球白菜的组培再生株在所测定的性状上保持了实生株的遗传稳定性。

Abstract

Brassica campestris ssp. pekinensis ‘AichiHakusai’ was introduced from Japan. Comparison of chromo some number, some morphological and biochemical characters was made between in vitro regenerated plants and seedling plants of ‘Aichi Hakusai’. The results indicated that the chromo some numbers of in vitro regenerated plants and seedling plants were the same(2n = 20). The growth of regenerated plants was not very uniform. at the early stage after transplanting. One month after transplanting, the growth of regenerated plants became uniform, and their morphological indexes were significantly higher than those of seedling plants under the same cultivating conditions. The change trends of soluble sugar and soluble protein contents in leaves were similar between regenerated plants and seedling plants. At the early stage after transplanting, soluble protein content was increased, carbon-nitrogen ratio(C /N)was low, and metabolic type was mainly nitrogen metabolism. As growth and development of plants proceeded, carbohydrate metabolism was enhanced with the increase of leaf area, resulting in the increase of both soluble sugar contents and C /N ratios. The results show that regenerated plants of ‘Aichi Hakusai’ retain the genetic stability of the seedling plants in the characters.

关键词爱知结球白菜; 再生植株; 染色体数目; 遗传稳定性;
Key wordsBrassica campestris ssp. pekinensis‘Aichi Hakusai’; regenerated plant; chromosome number; genetic stability
作者樊明琴,朱月林,朱丽华
所在单位南京农业大学园艺学院,江苏南京210095
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下载次数835
基金项目教育部留学回国人员科研启动基金资助项目 ( [ 2001 ]498);