香菇〔Lentinus edodes ( Berk.) Sing.〕子实体经热水提取, 乙醇沉淀, 得多糖粗品( Le) 。Le 脱游离蛋白, 脱色, 经DEAE-纤维素柱及纤维素凝胶柱层析分离纯化得到 Le-1 、Le-2 和Le-3 3 个级分。 经聚丙烯酰胺凝胶电泳和SepharoseCL-6B 柱层析鉴定为分子量分布均一的蛋白多糖。凝胶渗透色谱法测得 Le-1、 Le-2 和 Le-3 分子量分别为 954 000,90 000 和 14 000。3 个级分均由阿拉伯糖、木糖、甘露糖、半乳糖、葡萄糖和葡萄糖醛酸组成, 气相色谱测得 Le-1 的阿拉伯糖、 木糖、 甘露糖、 半乳糖、 葡萄糖的摩尔比为 0.39∶0.46∶1.00∶0.93∶14.13;Le-2 为 0.19∶0 .41∶1 .00∶0 .93∶10 .72;Le-3 为 .31∶0.47∶1.00∶1.15∶8.92。Le-1、Le-2 和 Le-3 葡萄糖醛酸含量( %) 分别为 24 .10、 34 .77 和 40.05, 蛋白质含量( %) 分别为 2.01 、 7.38 和 25.31, 红外扫描确定 Le-1 和 Le-2 的糖苷键为α型, Le-3 为β 型。
 

After being extracted with hot water and precipitated with alcohol, crude polysaccharide ( Le) was obtained from fruitbody of Lentinus edodes ( Berk .) Sing .Le was removed free proteins and colouring matter,then isolated and purified by DEAE-cellulose and cellulose gel column chromatography, three pure polysaccharides ( Le-1, Le-2, Le-3) were obtained .Results of polyacrylamide gel electrophoresis and Sepharose CL-6B column chromatography illustrate that they were protein-bond polysaccharides with homogeneous molecular weights . Molecular weights of Le-1, Le-2 and Le-3 were 954 000, 90 000 and14 000, respectively, by gel permeation chromatography .Three fractions were composed of arabinose (Ara) ,xylose ( Xyl) , mannose ( Man) , galactose ( Gal) , glucose ( Glc) and glucuronic acid .Gas chromatography illustrated that the neutral saccharides molar ratio of Le-1 was Ara∶Xyl∶Man∶Gal∶Glc =0 .39∶0 .46∶1 .00∶0 .93∶14 .13 ;Le-2 was Ara∶Xyl∶Man∶Gal∶Glc =0 .19∶0 .41∶1 .00∶0 .93∶10 .72 and Le-3 was Ara∶Xyl∶Man∶Gal∶Glc =0 .31∶0 .47 ∶1 .00 ∶1 .15 ∶8 .92 .Glucuronic acid contents ( %) of three fractions were 24 .10,34 .77 and 40 .05 and protein contents ( %) were 2 .01, 7 .38 and 25 .31, respectively .Infra-red spectrum showed that the glycosidic linkages of Le-1 and Le-2 were αtype, while that of Le-3 was β type.