2024年3月29日 星期五
香菇子实体蛋白多糖的分离纯化及组成结构分析
Isolation, purification and characterization of polysaccharides from the fruitbody of Lentinus edodes ( Berk. ) Sing.
2001年 第10卷 第2期 页码[4-6]    下载全文[0.5MB]  
摘要

香菇Lentinus edodes ( Berk.) Sing.〕子实体经热水提取, 乙醇沉淀, 得多糖粗品( Le) Le 脱游离蛋白, 脱色, DEAE-纤维素柱及纤维素凝胶柱层析分离纯化得到 Le-1 Le-2 Le-3 3 个级分经聚丙烯酰胺凝胶电泳和SepharoseCL-6B 柱层析鉴定为分子量分布均一的蛋白多糖凝胶渗透色谱法测得 Le-1Le-2 Le-3 分子量分别为 954 000,90 000 14 0003 个级分均由阿拉伯糖木糖甘露糖半乳糖葡萄糖和葡萄糖醛酸组成, 气相色谱测得 Le-1 的阿拉伯糖木糖甘露糖半乳糖葡萄糖的摩尔比为 0.390.461.000.9314.13;Le-2 0.190 .411 .000 .9310 .72;Le-3 .310.471.001.158.92Le-1Le-2 Le-3 葡萄糖醛酸含量( %) 分别为 24 .1034 .77 40.05, 白质含量( %) 分别为 2.01 7.38 25.31, 红外扫描确定 Le-1 Le-2 的糖苷键为α, Le-3 β
 

Abstract

After being extracted with hot water and precipitated with alcohol, crude polysaccharide ( Le) was obtained from fruitbody of Lentinus edodes ( Berk .) Sing .Le was removed free proteins and colouring matter,then isolated and purified by DEAE-cellulose and cellulose gel column chromatography, three pure polysaccharides ( Le-1, Le-2, Le-3) were obtained .Results of polyacrylamide gel electrophoresis and Sepharose CL-6B column chromatography illustrate that they were protein-bond polysaccharides with homogeneous molecular weights . Molecular weights of Le-1, Le-2 and Le-3 were 954 000, 90 000 and14 000, respectively, by gel permeation chromatography .Three fractions were composed of arabinose (Ara) ,xylose ( Xyl) , mannose ( Man) , galactose ( Gal) , glucose ( Glc) and glucuronic acid .Gas chromatography illustrated that the neutral saccharides molar ratio of Le-1 was AraXylManGalGlc =0 .390 .461 .000 .9314 .13 ;Le-2 was AraXylManGalGlc =0 .190 .411 .000 .9310 .72 and Le-3 was AraXylManGalGlc =0 .310 .47 1 .00 1 .15 8 .92 .Glucuronic acid contents ( %) of three fractions were 24 .10,34 .77 and 40 .05 and protein contents ( %) were 2 .01, 7 .38 and 25 .31, respectively .Infra-red spectrum showed that the glycosidic linkages of Le-1 and Le-2 were αtype, while that of Le-3 was β type.
 

关键词香菇; 蛋白多糖; 分离; 纯化; 性质
Key wordsLentinus edodes ( Berk . ) Sing.; protein-bond polysaccharides; isolation; purification; characterization
作者杨 娟1, 吴谋成2, 张声华2
所在单位1.贵州省·中国科学院天然产物化学重点实验室, 贵州 贵阳 550002;
2.华中农业大学食品科技系, 湖北 武汉 430070
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