2024年7月26日 星期五
细叶石斛有效成分分析及其水溶性提取物的血管舒张活性
Content analyses of polysaccharides, alkaloids and mineral elements from Dendrobium hancockii and vasodilation activity of its water-soluble extraction
2003年 第12卷 第1期 页码[6-9]    下载全文[0.5MB]  
摘要

对细叶石斛(Dendrobium hancockii Rofle)茎上部、茎中部、茎下部和根中的多糖、生物碱和矿质元素含量进行了分析,并研究了其水溶性提取物对SD大鼠胸主动脉血管的舒张效应。结果表明,细叶石斛中总多糖含量较高,并含有较多的钾、钙、镁、铁、锰、锶、钛和铜等元素,与细茎石斛〔D. moniliforme (L.)Sw.〕及铁皮石斛(D. officinale Kimura et Migo)相比,其水溶性多糖和生物碱的含量较低,而且不同部位中的含量存在差异。细叶石斛水溶性提取物强烈拮抗苯肾上腺素所致的大鼠胸主动脉血管收缩作用,对内皮完整和无内皮功能的2种样本均引起类似的浓度依赖性舒张效应,相关系数有显著意义(>r0.01),两者的IC50分别为0.309和0.404g /L。

Abstract

Contents of alkaloids, polysaccharides and mineral elements in different parts of Dendrobium hancockii Rofle and relaxed effects of its water-soluble extraction on isolated aortic rings of SD rats were researched. The experiment results showed that the medicinal plant D . hancockii has higher contents of total polysaccharides and mineral elements, while has relatively low in contents of alkaloids and water-soluble polysaccharides compared with Xijing Shi-hu 〔D. moniliforme ( L. )Sw . 〕 and Tiepi Shi-hu ( D. officinale Kimura et Migo).Distributions of these ingredients in upper, middle, lower stem and root parts in D. hancockii were unequal. The water-soluble extraction of D. hancockii evoked analogous concentration dependent relaxation on isolated aortic rings of SD rat with and without endothelium. The pharmacodynamic parameters indicated that the half inhibit concentration ( IC50)were about 0. 309 and 0. 404 g/L respectively.

关键词细叶石斛; 多糖; 生物碱; 微量元素; 血管舒张
Key wordsDendrobium hancockii Rofle; polysaccharides; alkaloids; mineral elements; vasodilation
作者陈云龙1 , 张 铭1 , 何国庆2 , 傅 琛3
所在单位1.浙江大学生命科学学院, 浙江 杭州 310012;
2.浙江大学生工食品学院, 浙江 杭州 310029;
3.浙江大学药学院,浙江 杭州 310031
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基金项目浙江省自然科学基金资助项目(301560);