应用组织培养技术对黄芩(Scutellaria baicalensis Georgi)进行多倍体诱导, 结果表明:组织培养条件下, 在培养基中添加一定浓度的秋水仙素, 或者把带有绿色芽点的黄芩愈伤组织经 0.2 %秋水仙素溶液浸泡一定时间后再进行培养, 均可诱发黄芩多倍体的产生, 但以后者效果较好, 诱导率可达 40.0% 。 通过试管苗根尖染色体显微观察, 鉴定出 50 多个黄芩同源四倍体, 为今后优良品种的选育打下基础。
 

A study of inducing polyploidy of Scutellaria baicalensis Georgi in the process of tissue culture was made. The results indicated that autotetraploid of S .baicalensis Georgi can be induced by adding colchicine in medium in tissue culture , or by immersing the callus of S .baicalensis Georgi in 0 .2 % colchicine solution for a period of time prior to culture .The later method w as proved to be a good way in producing autotetraploid , with an inducing ratio of 40 %. More than 50 lines of autotetraploid of S .baicalensis Georgi were determined by observing the chromosomes of obtained plantlets under microscope , and this laid dow n the foundation for the selecting and breeding of good varieties of S .baicalensis Georgi .