2024年7月22日 星期一
黄芩组织培养同源四倍体的诱导
The inducing of autotetraploid of Scutellaria baicalensis Georgi by tissue culture
2000年 第9卷 第1期 页码[9-11]    下载全文[0.4MB]  
摘要

应用组织培养技术对黄芩(Scutellaria baicalensis Georgi)进行多倍体诱导, 结果表明:组织培养条件下, 在培养基中添加一定浓度的秋水仙素, 或者把带有绿色芽点的黄芩愈伤组织经 0.2 %秋水仙素溶液浸泡一定时间后再进行培养, 均可诱发黄芩多倍体的产生, 但以后者效果较好, 诱导率可达 40.0% 。 通过试管苗根尖染色体显微观, 鉴定出 50 多个黄芩同源四倍体, 为今后优良品种的选育打下基础
 

Abstract

A study of inducing polyploidy of Scutellaria baicalensis Georgi in the process of tissue culture was made. The results indicated that autotetraploid of S .baicalensis Georgi can be induced by adding colchicine in medium in tissue culture , or by immersing the callus of S .baicalensis Georgi in 0 .2 % colchicine solution for a period of time prior to culture .The later method w as proved to be a good way in producing autotetraploid , with an inducing ratio of 40 %. More than 50 lines of autotetraploid of S .baicalensis Georgi were determined by observing the chromosomes of obtained plantlets under microscope , and this laid dow n the foundation for the selecting and breeding of good varieties of S .baicalensis Georgi .
 

关键词黄芩; 同源四倍体; 组织培养; 秋水仙素
Key wordsScutellaria baicalensis Georgi; autotetraploid; tissue culture; colchicine
作者陈柏君 ,高山林 ,卞云云
所在单位中国药科大学, 南京 210038
点击量1366
下载次数955