利用纸片法对蛋白核小球藻(Chlorella pyrenoidosa Chick.)脂溶性化合物的粗脂以及经硅胶柱层析分离后的不同组分进行了抑菌实验。结果表明,蛋白核小球藻的粗脂有极强的抑菌活性,其石油醚洗脱组分占总脂的63.9%,抑菌活性最弱;乙醇洗脱组分占总脂的32.2%,抑菌活性最强;苯洗脱组分只占总脂的3.9%,对玉米大斑病菌(Helminthosporium turcicum Pass)有极强的抑制活性。各洗脱组分及粗脂对革兰氏阴性菌均无抑制活性。成分分析结果表明,石油醚洗脱组分以烷烃类化合物为主,其中1- 十九烯烃含量最高;苯洗脱组分以6,10,14-三甲基-2-十五酮、十六碳酮和2-十一烷酮这3种酮类化合物为主;而乙醇洗脱组分含有大量的脂肪酸,其中γ-亚麻酸含量最高(56.673%)。蛋白核小球藻脂溶性化合物的抑菌活性是其各种化学成分相互综合作用的结果。

Crude grease and different elution fractions after the separation of the silica gel column chromatography in Chlorella pyrenoidosa Chick . were tested for the antimicrobial activities using the agar disc diffusion method. The crude grease has strong antimicrobial activities. The petroleum ether fraction occupied 63. 9 % of the total grease and has the most weak antimicrobial activities. The ethanol fraction occupied 32. 2% of the total grease and has the strongest antimicrobial activities. The benzene fraction only occupied 3. 9%of the total grease, but has stronger antimicrobial activity to Helminthosporium turcicum Pass. Every elution fractions and crude grease have no antimicrobial activity to Gram-negatie bacteria. The petroleum ether fraction was dominanted by hydrocarbon, in whih the content of 1-nonadecene is the highest. The benzene fraction was dominanted by 3 ketone compounds including 6, 10, 14-trimethyl-2-pentadecanone, Hexadecanone and 2-undecanone. The ethanol fraction has rich fatty acid, in which the content of γ -linolenic acid is the highest ( 56. 673 % ) . The antimicrobial activities of liposoluble compounds in C. pyrenoidosa were the results of the work in coordination with its various chemical compositions.