The essential oil was extracted from fresh leaf of Chamaecyparis pisifera (Sieb.et Zucc.) Endl. using steam distillation. Chemical components in the essential oil were identified by GC-MS and their relative contents were determined by peak area normalization. The results show that the extraction rate of the essential oil is 0.23%,and fifty-four compounds in the essential oil are identified. The main chemical components are cedrol,3,5-dimethyl-4-benzyl-isoxazole,(5α)-D-homoandrostan-17a-one,etc,and their relative contents are 32.32%,11.31% and 5.64%,respectively.