The salidroside content in different organs of wild plants of Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba from Xiaojin County of Aba Prefecture in Sichuan Province was analyzed by HPLC. The results show that there is an obvious difference in salidroside content among different organs of R. crenulata. And average content of salidroside in rhizome, root and stem is in order of 1. 529%, 1. 281% and 0. 131%, respectively. Salidroside is not detected in leaf, and that in tufted branching rhizome is highest (1. 828%) in rhizomes. It is suggested that only tufted branching rhizomes can be harvested when harvesting of R. crenulata.