This paper deals with the conditions of inducting callus and its differentia ting from young leaf of Saccharum arundinaceum Retz .The result shows that using MS medium added 2, 4-D 1 ～ 3 mg/ L is suitable.Various clone has obvious difference in callus induction and seedling differentiation .I t indicates that there are plentiful phy siological and genetic variations within the species.