The contents of total flavonoids and crude polysaccharides in leaf of Cyclocarya paliurus （Batal.） Iljinsk from four provenances in Zhangjiajie National Forest Park of Hunan Province， Tonggu County of Jiangxi Province， Xiushui County of Jiangxi Province， and Wuxishan Nature Reserve of Anhui Province in spring （May） and autumn （September） were compared. The results show that the content of total flavonoids in leaf of C. paliurus from each provenance in spring is significantly （P＜0.05） lower than that in autumn， and the average content of total flavonoids in leaf in spring （20.50 mg·g^-1） is significantly lower than that in autumn （22.38 mg·g^-1）； on the contrary， the content of crude polysaccharides in leaf of C. paliurus from each provenance in spring is higher than that in autumn， and the average content of crude polysaccharides in leaf in spring （30.84 mg·g^-1） is significantly higher than that in autumn （23.74 mg·g^-1）. Among four test provenances， the contents of total flavonoids and crude polysaccharides in leaf of C. paliurus from the provenance in Zhangjiajie National Forest Park of Hunan Province are both the highest， followed by the provenance in Tonggu County of Jiangxi Province. Overall， it is recommended that if the total flavonoids is exploitation target， the leaf of C. paliurus should be collected in September； while if the crude polysaccharides is exploitation target， the leaf of C. paliurus should be collected in May. In addition， the differences in contents of total flavonoids and crude polysaccharides in leaf of C. paliurus from different provenances are relatively great， and the provenances in Zhangjiajie National Forest Park of Hunan Province and Tonggu County of Jiangxi Province can be used as key research objects.