2024年12月22日 星期日
3 种海藻凝集素组分及糖抑制作用的初步研究
Preliminary studies on component and sugar inhibition of three marine algal lectins
2002年 第11卷 第4期 页码[19-21]    下载全文[0.4MB]  
摘要

3 种海藻( 铁钉菜 Ishige okamurae Yenda坛紫菜 Porphyra haitanensis Chang et Zheng 和脆江蓠 Gracilaria bursapastoris Silva) 中分离纯化得到铁钉菜凝集素( IOL) 坛紫菜凝集素( PHL) 及脆江蓠凝集素( GBL) 经测定, 它们分别
含有
7 %、14%11%的中性糖氨基酸组成中, 苯丙氨酸含量最高, 蛋氨酸和精氨酸次之3 种海藻凝集素的凝
血活性均可被单糖或双糖所抑制
, 在所测试的 13 种糖类中, 能够抑制 GBL 活性的糖类最多, 5 , 抑制 IOL 的糖
类最少
, 2 3 种糖蛋白( 胃粘蛋白卵粘蛋白和甲状腺球蛋白) IOL 没有抑制作用, 而卵粘蛋白能够抑制PHL
GBL 的凝血活性

 

Abstract

Three kinds of algal lectin ( IOL, PHL and GBL) were extracted and purified from three marine
algae (
Ishige okamurae Yenda, Porphyra haitanensis Chang et Zheng and Gracilaria bursa-pastoris Silva) .They contain 7 %, 14 %and 11 %neutral saccharide, respectively .In their amino acid composition, Phe hasthe highest content, followed by Arg or Met . Agglutinic activities of these lectins were inhibited with the presence of some mono- or bi-saccharides. In sugar inhibition, there were the most numbers of sugars which inhibited the agglutinic activity of GBL and the fewest numbers of sugars which inhibited the agglutinic activity of IOL .Three kinds of glycoproteins ( Mucin, Ovalbumin and Thyroglobuin) did not inhibit the activity of IOL, but Ovalbumin showed inhibition for the activities of PHL and GBL .
 

关键词海藻; 凝集素; 组分;
Key wordsmarine algae; lectin; component; sugar
作者郑 怡1, 余 萍2, 刘燕如1
所在单位1.福建师范大学生物工程学院, 福建 福州 350007;
2.福建师范大学高分子研究所,福建 福州 350007
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基金项目福建省自然科学基金资助项目( B0110009)