| 摘要 | 从 3 种海藻( 铁钉菜 Ishige okamurae Yenda、坛紫菜 Porphyra haitanensis Chang et Zheng 和脆江蓠 Gracilaria bursapastoris Silva) 中分离纯化得到铁钉菜凝集素( IOL) 、坛紫菜凝集素( PHL) 及脆江蓠凝集素( GBL) 。 经测定, 它们分别
含有 7 %、14%和 11%的中性糖。 氨基酸组成中, 苯丙氨酸含量最高, 蛋氨酸和精氨酸次之。3 种海藻凝集素的凝
血活性均可被单糖或双糖所抑制, 在所测试的 13 种糖类中, 能够抑制 GBL 活性的糖类最多, 达 5 种, 抑制 IOL 的糖
类最少, 仅 2 种。 3 种糖蛋白( 胃粘蛋白、卵粘蛋白和甲状腺球蛋白) 对 IOL 没有抑制作用, 而卵粘蛋白能够抑制PHL
和GBL 的凝血活性。
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| Abstract | Three kinds of algal lectin ( IOL, PHL and GBL) were extracted and purified from three marine
algae ( Ishige okamurae Yenda, Porphyra haitanensis Chang et Zheng and Gracilaria bursa-pastoris Silva) .They contain 7 %, 14 %and 11 %neutral saccharide, respectively .In their amino acid composition, Phe hasthe highest content, followed by Arg or Met . Agglutinic activities of these lectins were inhibited with the presence of some mono- or bi-saccharides. In sugar inhibition, there were the most numbers of sugars which inhibited the agglutinic activity of GBL and the fewest numbers of sugars which inhibited the agglutinic activity of IOL .Three kinds of glycoproteins ( Mucin, Ovalbumin and Thyroglobuin) did not inhibit the activity of IOL, but Ovalbumin showed inhibition for the activities of PHL and GBL .
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