The cell suspens ion cultures in leaves of Pueraria lobata (Willd. ) Ohwi seedlings were treated with salicylic acid (SA) , methyl jasmonate (MJ) and ethrel respectively. SA and MJ could cause cell brown, while ethrel had a positive effect on cell vitality. 0. 1 mg L SA and 1. 0 mg L MJ could promote the synthesis of puerarin and hence improved the yield. Ethrel, SA and MJ at higher concentrations restrained the product of puerarin. All these three substances had no effects on increasing cell biomass and puerarin release.