摘要 | 采用不同的生物活性测定方法比较了瑞香狼毒(Stellera chamaejasme L.)根部4种不同溶剂提取物的杀螨活性。结果表明,瑞香狼毒根提取物对山楂叶螨(Tetranychus viennensis Zacher)有很好的触杀和内吸活性。在触杀活性测试中,石油醚提取物和氯仿提取物的杀螨活性最高;在内吸作用中,乙醇、氯仿和石油醚提取物的杀螨活性均较高,杀螨效果显著。在对石油醚提取物的不同溶剂萃取物进行生物活性追踪测定中发现,石油醚萃取物和氯仿萃取物具有较高的生物活性,浓度为0.6 g.L-1,山楂叶螨的24 h校正死亡率分别达到93.22%和79.66%。 |
Abstract | The acaricidal activity of crude extracts from Stellera chamaejasme L. root using four different solvents was bioassayed by three bioassay methods. The results showed that extracts of S. chamaejasme root possessed better contact and systemic toxicity activities to Tetranychus viennensis Zacher. In contact activity test, the acaricidal activities of petroleum ether and chloroform. extracts were the highest. In systemic toxicity activity test, the acaricidal activities of alcohol, chloroform. and petroleum ether extracts were all higher. The results of bioassays of partitioned extracts of petroleum ether crude extracts by partitively extracting of four different solvents showed that the acaricidal activities of petroleum ether and chloroform. partitioned extracts were higher with adjusted mortalities (24h) of 93.22% and 79.66% respectively at concentration of 0.6g·L- 1. |
关键词 | 瑞香狼毒; 山楂叶螨; 杀螨活性; |
Key words | Stellera chamaejasme L.; Tetranychus viennensis Zacher; acaricidal activity |
作者 | 李 捷1,赵 飞1,刘素琪2,孔维娜1,师光禄2 |
所在单位 | 1.山西省农业科学院植物保护研究所,山西太原030031; 2.山西农业大学农学院,山西太谷030801 |
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基金项目 | 山西省资助回国留学人员科技项目(200485); |