采用化学发光法, 以 3 种活性氧 ROS(O^2÷、OH、H2O2)模型检测 5 种药用石斛及其培养物对 ROS 的清除作用。5 种药用石斛及其培养物的氯仿与甲醇提取物均具有清除3 种 ROS 的作用, 且多超过阳性对照 Vc 的清除能力。 氯仿提取物中以杯鞘石斛(Dendrobium gratiosissmum Rchb.f .)药材的清除 ROS 能力最强, 甲醇提取物中以铁皮石斛(D .officinale Kimura)培养物的清除 ROS 能力最强。 表明石斛是一类清除 ROS 效果较好的天然药物。
 

Using chemiluminescence system for detecting ability of scavenging reactive oxygen species ROS
(O^2÷-、·OH 、H2O2)in five species of Dendrobium Sw .The results show that most of chloroform. and methanol extracts from crude drugs and cultured tissues have significant scavenging effect on ROS and many of them are higher than positive control (Vc).The chloroform. extract from Dendrobium gratiosissmum Rchb .f .(crude drug)and methanol extract from D .officinale Kimura (cultured tissues)have the best scavenging of ROS ability .This results indicated that Dendrobium Sw .are good natural drugs for scavenging of ROS .