2024年10月31日 星期四
红凤菜新鲜茎叶中总黄酮提取物的 LC-MS 分析
LC-MS analysis of total flavonoids extracts from fresh stem and leaf of Gynura bicolor
2014年 第23卷 第3期 页码[108-110]    下载全文[0.7MB]  
Abstract

Four total flavonoids extracts were obtained from fresh stem and leaf of Gynura bicolor DC. by method of ethanol extraction-macroporous resin column chromatography-polyamide column  chromatography-recrystallization, and their compositions were analyzed by LC-MS technology. The results show that there are six groups of flavonoid absorption peak in total flavonoids extracts of G. bicolor, which is identified to be eight components including rutin, quercetin-3-O- rubinobioside, hyperoside, isoquercitrin, kaempferol-3-O-rubinobioside, kaempferol-3-O-rutinoside, kaempferol-3-O-galactoside and astragalin. This extraction method can effectively extract and purify total flavonoids from fresh stem and leaf of G. bicolor, so it can be used for factory production.

 

关键词红凤菜; LC-MS; 总黄酮; 槲皮素; 山柰酚
Key wordsGynura bicolor DC.; LC-MS; total flavonoids; quercetin; kaempferol
作者任冰如, 吕寒, 陈剑, 梁呈元, 吴菊兰, 李维林
所在单位江苏省• 中国科学院植物研究所(南京中山植物园) 江苏省药用植物研究开发中心 江苏省抗糖尿病药物筛选技术服务中心, 江苏 南京 210014
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基金项目江苏省产学研联合创新资金项目(BY2012213); 江苏省科技基础设施建设计划———科技公共服务平台项目(BM2011117)