为探究金属离子对绿绒蒿属(Meconopsis Vig.)3种植物花瓣呈色的影响，测定了盛开期全缘叶绿绒蒿〔M. integrifolia (Maxim.) Franch.〕、红花绿绒蒿（M. punicea Maxim.）和川滇绿绒蒿（M. wilsonii Grey-Wilson）花瓣中7种金属离子（包括Fe³⁺、Mg²⁺、Ca²⁺、K⁺、Mn²+、Cu²⁺和Zn²⁺）含量，对3个花期花瓣进行转录组分析。结果表明：盛开期，全缘叶绿绒蒿花瓣中7种金属离子含量显著(P<0.05)高于红花绿绒蒿和川滇绿绒蒿；红花绿绒蒿花瓣中Fe³+、Mg²⁺、K⁺、Mn²⁺和Zn²⁺含量显著低于川滇绿绒蒿，Cu²⁺和Ca²⁺含量显著高于川滇绿绒蒿。全缘叶绿绒蒿花瓣中，Fe³⁺、Fe²⁺和Cu²⁺相关GO富集条目以及镁螯合酶活性、钾离子稳态和对锌离子的反应条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中总体最多，锌离子结合和锰离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最少，螯合钙离子释放到细胞溶质条目中仅全缘叶绿绒蒿花瓣的差异unigene明显表达。红花绿绒蒿花瓣中，亚铁结合、镁离子结合、钙离子结合、钾离子结合、钾离子转运条目和锌离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最多，仅三价铁结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最少。川滇绿绒蒿花瓣中，细胞钙离子稳态和锰离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最多，Mg²⁺、K⁺和Cu²⁺相关GO富集条目以及亚铁结合、铁离子的细胞内螯合、对锌离子的反应、钙离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中总体最少。综上所述，推测Cu²⁺和Ca²⁺影响全缘叶绿绒蒿花瓣呈色，Fe²⁺、Mg²⁺、Mn²⁺、K⁺和Cu²⁺影响红花绿绒蒿花瓣呈色，Fe³⁺、Mg²⁺、Mn²⁺和Cu²⁺影响川滇绿绒蒿花瓣呈色。

In order to explore the effects of metal ions on the petal color of three species in Meconopsis Vig., contents of seven metal ions (including Fe³⁺, Mg²⁺, Ca²⁺, K⁺, Mn²⁺, Cu²⁺, and Zn²⁺) in petals of M. integrifolia (Maxim.) Franch., M. punicea Maxim., and M. wilsonii Grey-Wilson at the blooming stage were measured, and transcriptome analyses were conducted for petals at three flowering stages. The results show that at the blooming stage, contents of seven metal ions are significantly (P<0.05) higher in petals of M. integrifolia than in petals of M. punicea and M. wilsonii; contents of Fe³⁺, Mg²⁺, K⁺, Mn²⁺, and Zn²⁺ in petals of M. punicea  are significantly lower than those of M. wilsonii, and contents of Cu²⁺ and Ca²⁺ are significantly higher than those of M. wilsonii. In petals of M. integrifolia, evidently expressed differential unigenes in Fe³⁺-, Fe²⁺-, and Cu²⁺-related GO enrichment items, and magnesium chelatase activity, potassium homeostasis and response to zinc ion items are the most among three test species in Meconopsis in general, while those in zinc ion binding and manganese ion binding items are the least, and differential unigenes in the release of sequestered calcium ion into cytosol item only evidently express in petals of M. integrifolia. In petals of M. punicea, evidently expressed differential unigenes in ferrous iron binding, magnesium ion binding, calcium ion binding, potassium ion binding, potassium ion transport, and zinc ion binding items are the most among three test species in Meconopsis, and only those in ferric iron binding item are the least among three test species in Meconopsis. In petals of M. wilsonii, evidently expressed differential unigenes in cellular calcium ion homeostasis and manganese ion binding items are the most among three test species in Meconopsis, and those in Mg²⁺-, K⁺- and Cu²⁺-related GO enrichment items, and ferrous iron binding, intracellular sequestering of iron ion, response to zinc ion and calcium ion binding items are the least among three test species in Meconopsis in general. In conclusion, it is speculated that Cu²⁺ and Ca²⁺ affect the petal color of M. integrifolia, Fe²⁺, Mg²⁺, Mn²⁺, K⁺, and Cu²⁺ affect the petal color of M. punicea, and Fe³⁺, Mg²⁺, Mn²⁺, and Cu²⁺ affect the petal color of M. wilsonii.