采用茎尖组织培养技术,建立了杭白菊中大洋菊(Chrysanthemum morifolium Ramat.)的无菌试管苗体系。通过基本培养基和激素配比实验,筛选出杭白菊试管苗快速繁殖的最佳培养基组成。结果表明:最适宜的外植体为直径0.3mm的茎尖;诱导丛生芽的最适培养基为:MS+6-BA0.1mg·L^-1+IAA0.02mg·L^-1;诱导试管苗生根的最适培养基为:1/2MS+IAA0.7mg·L^-1。用电子显微镜进行病毒检测后,筛选出2个脱病毒株系,脱病毒试管苗可作为今后提供优质种苗的种源。

The shoot tip of Chrysanthemum morifolium Ramat.were used as explants to produce plantlets in vitro. A series of optimization experiments for cultural medium composition and concentration of phytohormones were investigated with a view to accelerate the propagation of virus-free materials. The results indicated that the most suitable explant was the shoot tip with diameter 0.3 mm. The optimized medium for crowd bud propagation was MS+ 6-BA0.1mg·L^{- 1}+ IAA0.0 2mg·L^-1.The optimized medium for rooting was 1/2MS+ IAA 0.7 mg·L^{- 1}. Virus-free materials were identified repeatedly under electron microscope and 2 virus-free strains were selected, which could be used as the sources for rapid-propagation.