Taking cotyledon and hypocotyl of Abelmoschus sagittifolius (Kurz.) Merr. as explants, the optimal hormone combinations for induction and proliferation cultures of their calli were selected. The results show that the growth status of callus is better with an induction rate of 100. 0% on MS medium (containing 5. 5 g·L^-1 agar and 30 g·L^-1 sugar, pH 6. 0) with 1. 0 mg·L^-1 2,4-D and 0. 1 mg·L^-1 KT, so this hormone combination is suitable for callus induction. And cotyledon is better than hypocotyl as explant for callus induction of the species. The effect of callus proliferation is the best with an average increment of 9. 74 g per culture bottle on MS medium with 2 mg·L^-1 6-BA and 1. 0 mg·L^-1 NAA, so this hormone combination is optimal for callus proliferation culture of A. sagittifolius cotyledon.