采用HPLC-ELSD法建立了茅苍术〔Atractylodes lancea (Thunb.)DC.〕水溶性成分的指纹图谱,并对不同产地野生与栽培茅苍术水溶性成分的指纹图谱进行了比较分析。结果表明,采用HPLC-ELSD法获得的茅苍术水溶性成分指纹图谱稳定、可靠、重复性好;供试茅苍术水溶性成分的保留时间一般在65 min之内,共有16个典型的共有色谱峰;供试茅苍术样品的指纹图谱与对照模式色谱图的相似度较高,说明该指纹图谱可作为茅苍术水溶性化学成分的特征指纹图谱。江苏产野生与栽培茅苍术水溶性成分的化学组成基本一致,以倍半萜苷类为主,主要成分均为苍术苷A;湖北产野生茅苍术水溶性成分的倍半萜苷类总含量较高,但主要成分不是苍术苷A。 

Fingerprints of water-soluble constituents in Atractylodes lancea (Thunb.)DC. Were established by HPLC-ELSD, and the fingerprints of water-soluble constituents in wild and cultivated A. lancea from different locations were compared. The results show that the fingerprint obtained by HPLC-ELSD is stable and reliable, and hasa good reproducibility. The retention time of water-soluble constituents in tested samples is less than 65min and the fingerprint has 16 typical common peaks. The fingerprints are quite similar to the standard chromatogram, indicating that the chromatogram fingerprint could beused as the characteristic fingerprint for the water-soluble constituentsin A. lancea. The chemical component of water-soluble constituents in wild A. lancea from Jiangsu Province are essentially identical with those in the cultivated ones which are mainly sesquiterpenoids, and the main component is atractyloside A.While water-soluble constituent in wild A. lancea from Hubei Province also contains a higher content of sesquiterpenoids, but the main component is not atractyloside A.