The contents of sweroside and isoorientin in Gentiana straminea Maxim. flower from ten locations of Qinghai Province were determined by HPLC. The results show that the content of isoorientin is higher than that of sweroside in G. straminea flower, and isoorientin and sweroside contents have certain differences among ten samples from ten locations. The content range of isoorientin and sweroside in ten samples is 0. 563% -0. 937% and 0. 346% -0. 453%, respectively. And isoorientin content in the sample from Xiangpishan and sweroside content in the sample from Xihaizhen are the highest. It is suggested that sweroside and isoorientin contents in G. straminea flower are affected by ecological environment of locations, and isoorientin content is affected more obviously.