采用正交实验设计,研究了诱导培养基中 6 - BA、NAA 和 V_C浓度及基本培养基类型 4 个因素对薄荷(Mentha haplocalyx Briq.)品种‘687’和‘沪 39’叶片愈伤组织诱导的影响,并研究了增殖培养基中 6 - BA、 NAA、 IBA 和2,4 - D 浓度对愈伤组织增殖的影响,筛选出适宜于品种‘687’和‘沪 39’叶片愈伤组织诱导和增殖的最适培养基。结果表明,培养基中 6 - BA、NAA 和 VC浓度及基本培养基类型对品种‘687’和‘沪 39’叶片愈伤组织的诱导率和褐化率均有一定的影响,但不同品种适宜的培养基配比有一定的差异;NAA 浓度对品种‘687’愈伤组织诱导的影响作用显著,影响品种‘沪 39’愈伤组织诱导的主要因素是 V_C浓度和基本培养基类型。 适宜于品种‘687’叶片愈伤组织诱导的培养基为含 0. 5 mg· L ^{- 1} 6 - BA 和 1. 5 mg· L ^{- 1} NAA 的 B5培养基,适宜于品种‘沪 39’ 愈伤组织诱导的培养基为含 1. 5 mg· L - 1 6 - BA、 1. 5 mg· L ^{- 1} NAA 和 100 mg· L ^{- 1} V_C 的 1 / 2MS 培养基, 均含 5. 5 g· L ^{- 1}琼 脂 和 30 g· L ^{- 1}蔗糖(pH 5. 5 ~ pH 5. 8)。 2 个品种叶片愈伤组织增殖所需的生长调节剂浓度及比例也有一定的差异,较高浓度的细胞分裂素有利于愈伤组织的增殖,品种‘687’ 和‘沪 39’叶片愈伤组织增殖的适宜培养基分别为 B5培养基和 1 / 2MS 培养基,均含 2. 0 mg· L ^{- 1} 6 - BA 和 1. 5 mg· L ^{- 1} NAA 以及 5. 5 g· L ^{- 1}琼脂和 30 g· L ^{- 1}蔗糖(pH 5. 5 ~ pH 5. 8)。

 

Effects of concentrations of 6-BA, NAA, V_C and type of basic medium in induction medium on induction of leaf callus from two cultivars ‘687’and ‘HU 39’ of Mentha haplocalyx Briq. were studied by orthogonal experiment and effects of concentrations of 6-BA, NAA, IBA and 2,4-D in multiplication medium on multiplication of the callus were also researched, and the best media for induction and multiplication of leaf callus from ‘ 687 ’ and ‘ HU 39 ’ were selected. The results show that the concentrations of 6-BA, NAA, V_C and type of basic medium have a certain influence on induction rate and browning rate of leaf callus, but the suitable proportion of medium for the two cultivars is not the same. The effect of NAA concentration on callus induction from ‘ 687 ’ leaf is significant, and VC concentration and basic medium type are the main factors affecting callus induction from ‘HU 39’ leaf .The best medium for callus induction of ‘687’leaf is B5 medium containing 0. 5 mg· L ^{- 1} 6-BA and 1. 5 mg· L ^{- 1} NAA, the best medium for that of ‘HU 39’leaf is 1 / 2MS medium containing 1. 5 mg· L ^{- 1} 6-BA, 1. 5 mg· L ^{- 1} NAA and 100 mg· L ^{- 1} V_C, both of the two media have 5. 5 g· L ^{- 1} agar and 30 g· L ^{- 1} sucrose ( pH 5. 5 - pH 5. 8). Amount of growth regulators needed for multiplication of leaf callus of the two cultivars is different, and a higher concentration of cytokinin is favorable for the multiplication of leaf callus. The best callus multiplication medium for ‘687’ and ‘ HU 39’ leaf is B5 medium and 1 / 2MS medium, respectively, and both of the two media contain 2. 0 mg· L ^{- 1} 6-BA, 1. 5mg· L ^{- 1} NAA, 5. 5 g· L ^{- 1} agar and 30 g· L ^{- 1} sucrose (pH 5. 5 - pH 5. 8).