Pollen morphology of Daphne genkwa Sieb. et Zucc. was observed by scanning electron microscope (SEM), and its viability and germination rate were measured by TTC and sucrose in vitro culture methods. The results show that pollen is stenopalynous type with a diameter of 15. 6-21. 6 μm. Per pollen has 10-16 apertures which is irregular circular with a diameter of 1. 4-2. 0 μm. Surface ornamentation of pollen is rough reticulate pattern which is circular polygon (tetragon-heptagon, mostly pentagon-hexagon). Pollen viability is 51% by TTC. Sucrose of different concentrations has a significant effect on pollen germination rate during pollen culture. And pollen germination rate is the highest with a percentage of 27. 0% in medium containing 50 g·L^-1 sucrose, while pollen could not germinate in medium containing sucrose over 250 g·L^-1 . Otherwise, there is the phenomenon of “multi-aperture germination”during pollen germinating.