The contents of six major constituents and total content of salvianolic acid from roots of seven species and one variety in Salvia L. collected from Yunnan Province were analyzed. The results showed that the contents of danshensu, protocatechuic acid, protocatechualdehyde, salvianolic acid B, tanshinone Ⅰ and tanshinone Ⅱ A reached to 392.55-3 844.30, 21.80-373.57, 16.442-180.360, 4 417.4-41 899.0, 82.20-2 893.70 and123.58-8 369.80 μg·g^-1, and total content of salvianolic acid also reached to 1.44%-4.53%.So the contents of constituents vary in different species and origin.