用0.05%～8.00%的甘露醇、山梨醇和聚乙二醇6000等3种渗透调节剂可提高转枯草芽孢杆菌纤溶酶(Bacillus subtilis fibrinolytic enzyme(BSFE))转基因烟草(Nicotiana tabacum L.)根系BSFE的分泌表达水平,其水培液BSFE活性在15d内基本呈抛物线型变化趋势。经3种渗透剂处理后转BSFE基因烟草水培液的BSFE活性峰值明显高于对照,且出现时间比对照相对延迟1-2d。甘露醇、山梨醇和聚乙二醇6000可作为该转基因烟草根系BSFE分泌表达的有效化学调节剂。

The different concentrations(0.05% -8. 00%)of penetrants such as mannitol, sorbitol and polyethylene glycol 6000 can increase the expression and secretion level of Bacillus subtilis fibrinolytic enzyme(BSFE) from root system of transgenic tobacco(Nicotiana tabacum L.). The BSFE activity in liquid culture medium took the parabolic change tendency within 15 d cultured. After treated by three penetrants, the peak values of BSFE activity was apparently higher than that of the control, and the peaks appeared 1-2 d later than that of the control. Mannitol, sorbitol and polyethylene glycol 6000 can be used as the effective chemical regulators for BSFE expression and secretion from the transgenic tobacco′s root system.