2024年7月24日 星期三
3种渗透剂对转基因烟草根系枯草芽孢杆菌纤溶酶(BSFE)分泌的调节
Regulation of three penetrants on secretion of Bacillus subtilis fibrinolytic enzyme from transgenic tobacco′s root system
2005年 第14卷 第2期 页码[1-5]    下载全文[0.7MB]  
摘要

用0.05%~8.00%的甘露醇、山梨醇和聚乙二醇6000等3种渗透调节剂可提高转枯草芽孢杆菌纤溶酶(Bacillus subtilis fibrinolytic enzyme(BSFE))转基因烟草(Nicotiana tabacum L.)根系BSFE的分泌表达水平,其水培液BSFE活性在15d内基本呈抛物线型变化趋势。经3种渗透剂处理后转BSFE基因烟草水培液的BSFE活性峰值明显高于对照,且出现时间比对照相对延迟1-2d。甘露醇、山梨醇和聚乙二醇6000可作为该转基因烟草根系BSFE分泌表达的有效化学调节剂。

Abstract

The different concentrations(0.05% -8. 00%)of penetrants such as mannitol, sorbitol and polyethylene glycol 6000 can increase the expression and secretion level of Bacillus subtilis fibrinolytic enzyme(BSFE) from root system of transgenic tobacco(Nicotiana tabacum L.). The BSFE activity in liquid culture medium took the parabolic change tendency within 15 d cultured. After treated by three penetrants, the peak values of BSFE activity was apparently higher than that of the control, and the peaks appeared 1-2 d later than that of the control. Mannitol, sorbitol and polyethylene glycol 6000 can be used as the effective chemical regulators for BSFE expression and secretion from the transgenic tobacco′s root system.

关键词枯草芽孢杆菌纤溶酶; 转基因烟草; 渗透调节; 根系分泌
Key wordsBacillus subtilis fibrinolytic enzyme(BSFE); transgenic tobacco (Nicotiana tabacum L.); osmoregulation; secretion of root system
作者王瑞刚1,2,张艳春2,张子义1,吴自荣2,王水平2
所在单位1.内蒙古农业大学生物工程学院,内蒙古呼和浩特010018;
2.华东师范大学生命科学学院,上海200062
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基金项目上海市科学技术委员会重大项目(9854190003);