Taking stem segments of oneyearold branch of Rhododendron molle ‘Jinzhizhu’ as explants， and using WPM medium （containing 6.5 g·L^-1 agar, pH 5.6） as basic medium， effects of medium proportion on differentiation of stem segment， proliferation of adventitious bud and rooting of plantlet were studied. The results show that differentiation rate of stem segment is the highest （83.3％） in induction medium （containing 6.5 g·L^-1 agar and 30 g·L^-1 sucrose， pH 5.6） with 1.0 mg·L^-1 2-ip and 0.1 mg·L^-1 NAA， and is significantly higher than that in other media； proliferation rate of adventitious bud is the highest （93.3％） in proliferation medium （containing 6.5 g·L^-1 agar， pH 5.6） with 1.0 mg·L^-1 2-ip， 0.05 mg·L^-1 NAA and 30 g·L^-1 sucrose or 1.5 mg·L^-1 2-ip， 0.10 mg·L^-1 NAA and 20 g·L^-1 sucrose， and proliferation multiple is also bigger. Rooting rate of plantlet is the highest in rooting medium （containing 6.5 g·L^-1 agar and 30 g·L^-1 sucrose， pH 5.6） with 0.50 mg·L^-1 NAA， which reaches 70.0％， but combined addition of NAA and IBA has no evident promotion effect on rooting of R. molle ‘Jinzhizhu’ plantlet.