Taking twenty flesh samplings of Cornus officinalis Sieb. et Zucc. collected from seven locations in four provinces as experimental materials, simultaneous determination of four active components of loganin, gallic acid, 5- hydroxymethylfurfural (5-HMF) and protocatechuic acid was carried out by the high performance liquid chromatographic method with diode array detection (HPLC-DAD). The results show that contents of loganin, gallic acid, 5-HMF and protocatechuic acid are 2. 632 9-7. 762 4, 0. 027 3-0. 360 9, 0. 003 9-0. 026 3 and 0. 158 1-0. 837 4 mg·g^-1 ,respectively. There are obvious differences in contents of four active components among samplings collected from different locations or among different samplings collected from same location, showing that it is related to environmental factors of locations.