Total flavonoids content in 98 grain samples of Hordeum vulgare Linn. collected from 22 provinces and cities and its processed products (including raw malt, torrefied malt and ustulate malt) were determined by visible spectrophotometry method, and also, effects of row types and seed coat bare situations on total flavonoids content in grain and its processed products were also studied. The results show that there are bigger differences in total flavonoids content among grain samples and its processed products from different sources, and generally, total flavonoids contents in grain, raw malt and torrefied malt of hull barley “Leqing heshenqiao damai”from Zhejiang are higher. The average of total flavonoids content in grain, raw malt, torrefied malt and ustulate malt of H. vulgare are 3. 23, 4. 47, 5. 41 and 6. 16 mg·g^-1 , respectively with extremely significant difference, meaning that processing method has an extremely significant effect on total flavonoids content of H. vulgare. Generally, different row types and seed coat bare situations have no significant effect on total flavonoids content in grain and its processed products of H. vulgare.