2024年5月27日 星期一
香石竹叶片离体再生体系的建立
Establishment of regeneration system from leaf explant of D ianthus caryophyllus
2005年 第14卷 第1期 页码[16-19]    下载全文[0.6MB]  
摘要

以香石竹(Dianthus caryophyllus Linn. )无菌苗叶片为外植体,从不同细胞分裂素及其他激素配合使用等方面进行筛选,建立香石竹叶片离体再生体系。结果表明,不同的细胞分裂素影响叶片不定芽分化频率,其中较低浓度的 6-BA(0.5mg·L-1 )和TDZ(0.001mg·L-1 )配合使用能有效诱导香石竹叶片不定芽分化;添加一定浓度的PP333 (4mg·L-1 )可提高叶片不定芽分化频率和平均芽数。香石竹叶片不定芽分化的适宜培养基为:MS+0.002mg·L-1 TDZ+0. 5mg·L-1 6-BA+0. 2mg·L-1 IAA+4mg·L-1 PP333;壮苗培养基为:MS+0. 2mg·L-1 6 BA+0.2mg·L-1 IAA;生根培养基为: 1/2MS。不定芽诱导频率达到 42. 61%,平均芽数为 4.53个。

Abstract

The regeneration system from leaf of Dianthus caryophyllus Linn. was established by using different cytokinins combining with other phytohormones. The results showed that the kinds of cytokinin effected the differentiation rate of adventitious buds from leaf; 6-BA and TDZ in relatively low concentration can induce differentiation of adventitious buds from leaf and PP333 can increase the differentiation rate and the number of adventitious buds. The system of plant regeneration from leaf of D. caryophyllus consisted of: MS+ 0.002 mg·L-1 TDZ+ 0. 5 mg·L-1 6-BA+ 0. 2 mg·L-1 IAA+ 4 mg·L-1 PP333for the differentiation of adventitious buds, MS+ 0. 2 mg·L-16-BA+ 0. 2 mg·L-1 IAA for the growth of adventitious buds and 1/2 MS for the rooting of adventitious buds. The differentiation rate of adventitious buds is 42.61% and the average number of adventitious bud is 4.53.

关键词香石竹; 叶片; 不定芽分化; 再生体系;
Key wordsDianthus caryophyllus Linn.; leaf; differentiation of adventitious bud; regeneration system
作者陈英1,王光萍1,诸葛强1,蔡有铭2,黄敏仁1,王明庥1
所在单位1.南京林业大学林木遗传和基因工程实验室,江苏南京210037;
2.上海市农业委员会,上海200001
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基金项目国家转基因专项(JY B 29 02)资助项目;