2024年7月23日 星期二
石荠苧精油的GC-MS分析及其抑菌活性的研究
Studies on chemical component and antibacterial activity of essential oil from Moslas cabra
2006年 第15卷 第3期 页码[26-30]    下载全文[0.6MB]  
摘要

利用水蒸汽蒸馏法提取石荠苧〔Mosla scabra(Thunb.) C. Y. Wu et H. W. Li〕精油,得油率为1.2%。采用GC-MS方法分析了该挥发油的化学组成,检测到125个峰,共鉴定出其中67个化学成分并测定其相对含量,占总含量的95.25%。其中,(R)侧柏酮(26.11%)、(M)侧柏酮(13.66%)、石竹烯(8.89%)、桉叶油素(7.99%)、(R)-α-石竹烯(6.45%)、芹菜脑(5.43%)和β-荜澄茄油烯(4.51%)为主要成分。体外抑菌实验表明,该精油对7种供试细菌均有抑制作用,对金黄色葡萄球菌(Staphylococcus aureus Roenbach)、普通变形杆菌(Proteas vulgaris Hauser)、甘薯青枯假单胞菌(Ralstonia soanacearum E. F. Smith)、大肠杆菌(Escherichia coli Castellani et Chalmers)和短小芽孢杆菌(Bacillus pumilus Meyer et Gottheil)的最低抑菌浓度(M IC)为2%,对枯草芽孢杆菌(Bacillus subtilis Cohn)和藤黄八叠球菌(Sarcina lutea Schroeter)的M IC为1%。

Abstract

The essential oil of Mosla scabra (Thunb.)C. Y. Wu et H. W. Li was extracted by water-steaming method. The extraction rate was 1.2%. The chemical components were analyzed by means of GC-MS. Sixty-senven components, 95. 25% of total content, had been identified from 125 separated peaks.The main components were (R) thujone (26.11%), (M) thujone (13.66%), caryophyllene (8.89%), eucalyptol(7.99%), (R)-α-caryophyllene(6.45%), apiol(5.43%)and β-cubebene(4.51%). The antibacterial experiments were carried on 7 tested strains in vitro, which showed that the oil had a certain antibacterial activity. The minimum inhibitory concentration (MIC) for Staphylococcus aureus Roenbach, Proteas vulgaris Hauser, Ralstonia soanacearum E. F. Smith, Escherichia coli Castellani et Chalmers and Bacillus pumilus Meyer et Gottheil was 2%, and that for Bacillus subtilis Cohn and Sarcina lutea Schroeter was 1%.

关键词石荠苧; 精油; 化学成分; 抑菌活性; 最低抑菌浓度(MIC);
Key wordsMosla scabra (Thunb.) C. Y. Wu et H. W. Li; essential oil; chemical component; antibacterial activity; minimum inhibitory concentration(MIC)
作者吴翠萍,吴国欣,陈密玉,林燕妮,黄鹭强
所在单位福建师范大学生命科学学院,福建福州350007
点击量1143
下载次数815
基金项目福建省自然科学基金资助项目(B0210009);