采用超高效液相-四级杆-飞行时间串联质谱技术（UPLC-Q-TOF/MS）检测5个产地菊三七〔Gynura japonica （Thunb.） Juel.〕根中6个吡咯里西啶生物碱（PAs）成分的含量差异。结果显示：不同产地菊三七根中PAs总含量差异显著（P＜0.05），其中，云南昆明产菊三七根中的PAs总含量最高（3 107.12 μg·g^-1），而四川乐山产菊三七根中的PAs总含量最低（310.47 μg·g^-1）。在5个产地菊三七根中均检出千里光菲灵N-氧化物和千里光宁N-氧化物。除湖北恩施外，其余产地菊三七根中并未检出全部6个PAs成分。研究结果显示：不同产地菊三七根中PAs的种类和含量均存在较大差异。

The differences of contents of six pyrrolizidine alkaloids （PAs） in roots of Gynura japonica （Thunb.） Juel. from five origins were detected by using ultra performance liquid chromatography-quadrupoletime of flight-mass spectrometer （UPLC-Q-TOF/MS）. The results show that there are significant （P<0.05） differences in total contents of PAs in roots of G. japonica from different origins， in which， total content of PAs in roots of G. japonica from Kunming of Yunnan is the highest （3 107.12 μg·g^-1）， while that in roots of G. japonica from Leshan of Sichuan is the lowest （310.47 μg·g^-1）. Seneciphylline N-oxide and senecionine N-oxide are detected in roots of G. japonica from all five origins. Except for Enshi of Hubei， not all six PAs are detected in roots of G. japonica from the other origins. It is suggested that there are great differences in types and contents of PAs in roots of G. japonica from different origins.