Effects of methanol extracts from barks， branches and leaves and 15β-hydroxy-ent-16-kaur-19-oic-β-D-glucopyranosyl ester （Gd-1） of Gochnatia decora （Kurz） A. L. Cabrera on viability of mouse RAW264.7 macrophages and production of NO， reactive oxygen species （ROS）， tumor necrosis factor-α （TNF-α）， and interleukin-6 （IL-6） in mouse RAW264.7 macrophages were studied by using the model of lipopolysaccharide （LPS）induced mouse RAW264.7 macrophages. The results show that 0.39， 1.56， 6.25， 25 and 100 μg·mL^-1 methanol extracts from barks， branches and leaves， and 0.39， 1.56， 6.25， 25 and 100 μmol·L^-1 Gd-1 of G. decora have no obvious effect on growth of mouse RAW264.7 macrophages； compared with the model control group， methanol extracts from barks， branches and leaves and Gd-1 of G. decora can inhibit NO and ROS production， and high dosage group has significant effects on TNF-α and IL-6. It is suggested that methanol extracts from barks， branches and leaves and Gd-1 of G. decora all have some anti-inflammatory activities， and Gd-1 can serve as a target product for further isolation， purification， and activity study.