Total ginkgolic acids content in leaf of fruit-utilization type of Ginkgo biloba L. from 17 locations in 12 provinces and with different tree ages from 10 to 100 a were analyzed by HPLC. The results show that total ginkgolic acids content in G. biloba leaf varies with changing of locations and tree ages. Total ginkgolic acids content in 10-30 year-old G. biloba is all lower (mass ratio 0. 81% -0. 93% ), in which that in 25 year-old G. biloba is the lowest (mass ratio 0. 81% ), but that in 50 and 100 year-old G. biloba is higher with a mass ratio of 1. 20% and 1. 07% , respectively. Total ginkgolic acids content in G. biloba leaf from Changting of Fujian is the highest (mass ratio 2. 80% ), and that from Jiangsu, Shandong,Anhui and Zhejiang is all lower (mass ratio 0. 51% -0. 99% ), showing a certain regional distribution characteristics.