2024年10月31日 星期四
RP-HPLC法测定怀槐中染料木素和芒柄花黄素含量
RP-HPLC determination of genistein and formononetin in various parts of Maackia amurensis
2004年 第13卷 第2期 页码[60-61] 下载全文[0.3MB]
Abstract | A method for the determination of genistein and formononetin in Maackia amurensis Rupr. et Maxim. by HPLC was reported. The RP-HPLC methods were used as follows: Hypersil ODS C18 column; The mobil phase is MeOH-1% acetic acid solution(gradient), genistein and formononetin were determined by UV detector at 261nm and 249nm respectively. The results showed that average recovery and precision (RSD) for genistein and formononetin were 100.4%,\ 101.8%and2.65%, 2.16% respectively. The method is appropriate for determinaton of genistein and formononetin in M. amurensis.
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关键词 | 怀槐; 反相高效液相法; 染料木素; 芒柄花黄素; |
Key words | Maackia amurensis Rupr. et Maxim.; RP-HPLC; genistein; formononetin |
作者 | 黄文哲,韦佳,王峥涛,周荣汉 |
所在单位 | 中国药科大学,江苏南京210038 |
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