A method for the determination of genistein and formononetin in Maackia amurensis Rupr. et Maxim. by HPLC was reported. The RP-HPLC methods were used as follows: Hypersil ODS C₁₈ column; The mobil phase is MeOH-1% acetic acid solution（gradient）, genistein and formononetin were determined by UV detector at 261nm and 249nm respectively. The results showed that average recovery and precision （RSD） for genistein and formononetin were 100.4%,\ 101.8%and2.65%, 2.16% respectively. The method is appropriate for determinaton of genistein and formononetin in M. amurensis.