Water content, dry weight per plant and total flavonoids content in different parts of Dicranopteris pedata (Houtt.) Nakai at different collection times (from April to December) were comparatively analyzed. The results show that at different collection times, there are extremely significant differences in water content and dry weight per plant, and also there are obvious differences in total flavonoids content in rhizome, leaf, lower and upper stems. Generally, from mid April, water content of D. pedata begins to decrease gradually and reaches the lowest in December. Dry weight per plant increases generally and reaches the highest in mid October with a value of 155. 41 g. Total flavonoids content in rhizome and leaf are extremely significantly (P<0. 01) higher than that in stem and their maximum are 28. 53% and 23. 43% , respectively, while that in upper and lower stems are only 8. 17% and 9. 39% , respectively. Comprehensively considering on many factors such as total flavonoids content, medicinal material yield and collection time, etc, the optimal raw material for extracting total flavonoids is confirmed to be leaf, the optimal collection time to be mid October.