2024年2月25日 星期日
江浙地区杨梅主要品种的ISSR分析
Analysis on ISSR marker of prmiary cultivars of waxberry in Jiangsu and Zhejiang
2006年 第15卷 第3期 页码[17-20]    下载全文[0.5MB]  
摘要

为研究江浙地区杨梅(Myrica rubra Sieb. et Zucc.)的亲缘关系,运用ISSR-PCR分析方法对其主要栽培品种进行了分析。8条引物扩增出58个DNA片段,其中多态性片段42个,占总扩增片段的72.4%。结合遗传距离分析,运用UPGMA法构建了分子系统树。结果表明,所分析的14个品种和1个优良单株以0.14为结合线可分为5组,说明江浙地区的杨梅在广泛基因交流的情况下,存在局部的隔离。‘小叶细蒂’与‘极早熟1号’亲缘关系较近,前者可能是后者的亲本之一。 

Abstract

Inter-simple sequence repeat(ISSR)was used to analysis genomic DNA variations and genetic relationships of waxberry(Myrica rubra Sieb. et Zucc.) in Jiangsu and Zhejiang provinces. 8 of11 ISSR primers were proved to be polymorphic and informative. Total 58 DNA fragments were amplified and 42 were polymorphic(72. 5%). The genetic distance was analysed according ISSR result and a dendrogram was constructed using UPGMA method. All cultivars tested were classified into 5 groups and the excellent individual (Jizaoshu No.1) has a close relationship with ‘Xiaoyexidi’.  This  indicates that there were a wide gene flow and topical isolation between waxberry cultivars of two provinces. Maybe ‘Xiaoyexidi’ was a parent of ‘JizaoshuNo. 1’.

关键词杨梅; ISSR; 品种鉴定; 亲缘关系
Key wordswaxberry(Myrica rubra Sieb. et Zucc.); ISSR; cultivar identification; genetic relationship
作者钱剑林1,2,俞文生3,王化坤2,3,娄晓鸣2,章 镇2
所在单位1.苏州农业职业技术学院,江苏苏州215008;
2.南京农业大学园艺学院,江苏南京210095;
3.江苏省太湖常绿果树技术推广中心,江苏苏州215107
点击量1086
下载次数740