以江苏东台产薄荷(Mentha haplocalyx Briq.)的茎尖为外植体,以MS为基本培养基,研究了培养基添加物和培养条件对薄荷试管苗玻璃化现象的影响。结果显示,导致薄荷玻璃化苗产生的主要因素是培养基中的6-BA、蔗糖和琼脂浓度以及培养温度和光照时间;当6-BA浓度为2mg·L^-1、蔗糖浓度为4%、琼脂浓度为0.70%、培养温度25℃、光照12h·d^-1 (2 000 lx)时,薄荷试管苗的繁殖系数较高,玻璃化苗率较低。

The shoots of Mentha haplocalyx Briq. from Dongtai of Jiangsu Province were cultured in MS medium to observe the influence of additives and culture condition on vitrification of plantlets. The results showed that the main factors leading to vitrification of M. haplocalyx plantlets were concentrations of  6-BA, sucrose and agar in the culture medium, culture temperature and irradiation time. Under the condition of 2mg·L^-1 6-BA, 4%sucrose, 0.70% agar and culture temperature 25℃ , irradiation time 12h·d^-1(2000lx), the coefficient of proliferation is higher, the rate of vitrification is lower.