2024年4月27日 星期六
人参毛状根培养过程中对活性氧清除能力的动态变化
Dynamic change of scavenge reactive oxygen species ( ROS)of hairy root of Panax ginseng during its culture process
2002年 第11卷 第4期 页码[22-24]    下载全文[0.4MB]  
摘要

采用化学发光法 ,考察了人参 (Panax ginseng C .A .Mey)毛状根培养过程中对 3种重要活性氧ROS(O2-·、OH·、H2O2 )清除能力的动态变化 ,发现在毛状根生长对数期时对 3种ROS都有很强的清除能力 ,而在生长停滞期较低 ,符合人参毛状根生长的S型曲线 ,印证了人参毛状根的生长和衰老过程。

Abstract

Dynamic changes of scavenging reactive oxygen species ROS (O2-·、OH·、H2O2)in hairy root of Panax ginseng C.A . Mey ( HRPG)were studied during its culture process by luminescence. The results showed that HRPG has significant scavenging effect on ROS at the logarithmic multiplication phase but the effect decreased after the logarithmic multiplication phase. The results coincide with growth ‘S’ curve of HRPG and show growth and aging process of HRPG .

关键词人参毛状根; 活性氧; 化学发光法
Key wordshairy root of Panax ginseng C .A.Mey (HRPG); reactive oxygen species (ROS); luminescence
作者刘 峻 , 丁家宜 , 周倩耘
所在单位中国药科大学中药学院生物技术研究室, 江苏 南京 210038
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