Terpene lactone contents in different organs of Ginkgo biloba L. and leaf, stem and root of G. biloba seedlings treated with chlorocholine chloride were determined by GC. The results showed that total terpene lactone contents in the order from high to low were bud (1.973 mg·g^-1), leaf (1.753 mg·g^-1), root, stem, and the terpene lactone content in phloem was higher than that in xylem. Leaf contained more bilobalide than root and stem. Chlorocholine chloride treatment significantly increased terpene lactone content of G. biloba seedlings. The possible way of terpene lactone biosynthesis was discussed.