摘要 | 为了探究葱莲〔Zephyranthes candida (Lindl.) Herb.〕在生物农药上的应用价值,采用水浴加热浸提法提取野生葱莲地上部和地下部的总生物碱,并比较质量浓度500 μg·mL-1地上部和地下部总生物碱提取物对常见植物病原菌(包括12种植物病原真菌和4种植物病原细菌)的抑制率。结果表明:葱莲地上部和地下部总生物碱的提取率分别为18.241%和9.725%。葱莲地上部和地下部总生物碱提取物对稻瘟病菌(Magnaporthe oryzae)的抑制率均较高,分别为75.29%和82.62%;2种提取物对其余植物病原真菌和植物病原细菌的抑制率均较低,多在20%以下。并且,葱莲地上部和地下部总生物碱提取物对多数植物病原菌的抑制率无显著差异。研究结果显示:葱莲地上部和地下部总生物碱提取物具有防治稻瘟病的潜力,且地上部总生物碱提取物的使用成本更低。 |
Abstract | In order to explore the application value of Zephyranthes candida (Lindl.) Herb. in biopesticides, the total alkaloids from above- and under-ground parts of wild Z.candida were extracted by using a water-bath heating extraction method, and the inhibition rates of total alkaloid extracts from above- and under-ground parts with mass concentration of 500 μg·mL-1 on common phytopathogens (containing 12 plant-pathogenic fungi and 4 plant-pathogenic bacteria) were compared. The results show that the extraction rates of total alkaloids from above- and under-ground parts of Z. candida are 18.241% and 9.725%, respectively. The inhibition rates of total alkaloid extracts from above- and under-ground parts of Z. candida on Magnaporthe oryzae are relatively high, which are 75.29% and 82.62%, respectively; the inhibition rates of the two extracts on the other plant-pathogenic fungi and plant-pathogenic bacteria are relatively low, which are mostly lower than 20%. In addition, there is no significant difference in inhibition rates of total alkaloid extracts from above- and under-ground parts of Z. candida on most phytopathogens. It is suggested that the total alkaloid extracts from above- and under-ground parts of Z. candida have the potential for preventing and controlling rice blast, and the usage cost of total alkaloid extracts from above-ground part is lower. |
关键词 | 葱莲; 总生物碱; 植物病原菌; 抑制率; 生物农药 |
Key words | Zephyranthes candida (Lindl.) Herb.; total alkaloids; phytopathogen; inhibition rate; biopesticide |
作者 | 乔思薇1,2a,2b, 姚静远1,2a,2b, 印敏2a,2b, 王忠2a,2b, 王奇志1,2a,2b, 冯煦2a,2b, 陈雨2a,2b, 徐曙2a,2b |
所在单位 | 1. 南京中医药大学, 江苏 南京 210023; 2. 江苏省中国科学院植物研究所(南京中山植物园): a. 江苏省植物资源研究与利用重点实验室, b. 江苏省中药材生态种植与高值化利用工程研究中心, 江苏 南京 210014 |
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基金项目 | 江苏省中国科学院植物研究所自主科研重点项目(BM2018021-5) |