2024年2月25日 星期日
银杏不同器官蛋白质动态变化的电泳分析
Analysison dynamic changes of protein in different organs of Ginkgo biloba by SDS-PAGE
2007年 第16卷 第2期 页码[32-35]    下载全文[0.6MB]  
摘要

利用SDS-PAGE电泳技术对不同季节银杏(Ginkgo biloba L.)雌雄株叶片和枝条中的贮藏蛋白质组分和可溶性蛋白质含量进行了研究。结果表明,银杏叶片中的可溶性蛋白质含量从10月中旬开始逐渐减少,11月中旬降到最低;枝条中的可溶性蛋白质含量从秋末到整个冬季逐渐增加,进入早春后大幅度下降;同一季节内,叶片中的蛋白质含量明显高于枝条,且雄株叶片和枝条的蛋白质含量高于雌株。单向电泳结果表明,银杏雌雄株叶片中相对分子质量为55 000和32 000的蛋白质谱带均于11月中旬前消失,表明这些蛋白质在银杏落叶前发生了转移。在银杏雌雄株枝条中,相对分子质量为59 000、32 000和27 000的蛋白质谱带均在12月底染色最深,进入早春后消失;雄株枝条中相对分子质量为18 000的蛋白质谱带在12月底前稳定出现,进入早春后消失。表明银杏枝条中的这些蛋白质均表现出冬季积累而春季降解的动态过程。 

Abstract

The protein fraction and soluble protein content in leaf and branch of Ginkgo biloba L. were studied by SDS-PAGE technique. The results showed that soluble protein content in leaf of male and female trees of G. biloba began to decrease at the second ten-days of October, and to the  lowest level at the second ten-days of November. While soluble protein contentin branch increased gradually from the late of autumn to the late of winter, and then decreased sharply in the early of next spring. In the same season, soluble protein content in leaf was higher than that in branch, and that of male tree was higher than that of female tree. The SDS-PAGE bands of MW (relative molecular weight)55 000 and MW 32 000 proteins in leaf could be observed until the second ten-days of November and then disappeared, indicating that these proteins were trans formed before leaves falling down. The darkest bands of MW 59 000, MW 32 000 and MW 27 000 proteins in branch were observed before the end of December but disappeared in the early of next spring; MW 18 000 protein band could be observed only male tree and maintained stably before the end of December and disappeared in the early of next spring. It is suggested that these proteins in G. bilobab ranch would be accumulated in winter and decomposed in spring.

关键词银杏; 可溶性蛋白质; SDS-PAGE;
Key wordsGinkgo biloba L.; soluble protein; SDS-PAGE
作者王改萍,彭方仁
所在单位南京林业大学森林资源与环境学院,江苏南京210037
点击量960
下载次数654
基金项目国家自然科学基金资助项目(30371154);