2022年1月21日 星期五
不同种源太子参的RAPD分析
PAPD analysis on Pseudostellaria heterophylla from different provenances
2007年 第16卷 第3期 页码[19-22]    下载全文[0.5MB]  
摘要

应用RAPD标记方法分析了15个太子参〔Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.〕种源间的遗传多样性和亲缘关系。10条随机引物共扩增出65条带,其中多态性条带37条,多态性条带百分率达56.9%。用聚类分析方法可将15个太子参种源分为4类;地理分布越近,太子参种源间的遗传差异越小。来源于安徽宣城的太子参种源遗传变异明显,辽宁凤城的野生太子参与山东地区的太子参栽培种源间的亲缘关系较近,与江苏各地太子参种源的亲缘关系则较远,这些种源均可作为育种材料。自然环境,尤其是生态环境的变化,对太子参的遗传变异有一定的影响。 

Abstract

Genetic diversity and phylogenetic relationship of fifteen provenances of Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm. are analyzed by RAPD technique. The results show that 65bands are amplified by ten random primers, and there are 37polymorphic bands which account for 56.9% of total bands. Fifteen provenances of P. heterophylla are dividedinto 4groups according to cluster analysis result of RAPD bands. The closer the geographic distances of P. heterophylla provenances, the less their genetic differences. The provenance from Xuancheng of Anhui Province has evident genetic variation, and the wild provenance from Fengcheng of Liaoning Province has a close phylogenetic relationship with that from Shandong Province while has a far phylogenetic relationship with that from Jiangsu Province, so these provenances can beconsidered as breeding materials. The natural environment, especially localec ological conditions, also shows some influences on genetic variations among different provenances of P .heterophylla.

关键词太子参; DNA随机扩增多态性; 亲缘关系;
Key wordsPseudostellaria heterophylla (Miq.)Pax ex Pax et Hoffm.; RAPD; phylogenetic relationship
作者朱 艳1,秦民坚1,杭悦宇2,郑晓清1
所在单位1.中国药科大学中药资源教研室,江苏南京210038;
2.江苏省•中国科学院植物研究所(南京中山植物园),江苏南京210014
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基金项目江苏省自然科学基金资助项目(BK2005206); 中国药科大学校基金资助项目(B0608);