2024年6月17日 星期一
四甲基氯化铵在杨树 RAPD 扩增反应中的作用
The effect of tetramethyl ammonium chloride on RAPD amplification in poplar
2001年 第10卷 第2期 页码[11-13]    下载全文[0.5MB]  
摘要

根据 20 个不同 RAPD 随机引物对杨树 DNA 的扩增结果, 对四甲基氯化铵( TMACI) 在杨树 RAPD 扩增反应中的作用进行了研究, 发现 TMACI 可明显改善杨树的 RAPD 扩增效果, 因此 TMACI 可作为一种有效成分加入杨树RAPD 反应体系中同时比较了不同浓度的 TMACI 对杨树 RAPD 扩增反应的影响, 确定最适合杨树 RAPD 扩增反应TMACI 浓度为 10 50 μmol /L利用 3 个具亲缘关系的杨树家系对含 TMACI RAPD 反应体系的实验结果进行验证, 证实该体系获得的遗传信息是可靠的
 

Abstract

According to the amplification result in poplar by 20 10-mer arbitrary primers, the effect of tetramethyl ammonium chloride ( TMACI) on RAPD amplification in poplar was studied .It revealed that the effect of TMACI on RAPD amplification is significant and which can improve the result of RAPD amplification .TMACI can be used as an effective composition for assembling the RAPD reaction .The effect of different concentration of TMACI on RAPD amplification was also studied and the suitable concentration ( 10 -50μmol/ L) of TMACI used in the RAPD reaction for poplar was determined .In order to test the new reaction system, three pedigrees with clear relationship were adopted, and the result showed that the new system is reliable for genetic study .
 

关键词四甲基氯化铵; RAPD; 扩增反应特异性; 杨树
Key wordsTMACI; RAPD; speciality of amplified action; poplar
作者尹佟明, 李淑娴, 郑阿宝, 王明庥
所在单位南京林业大学, 江苏 南京 210037
点击量1023
下载次数732
基金项目国家自然科学基金资助项目( 39770628) ;Supported by the International Foundation for Science of Sweden to Dr Yin ( No .D 2997-1)