The effects of substrates complemented with different particle sizes and proportions of porous soil amendments on ex vitro rooting of stem segments of tissue culture seedlings of Vaccinium corymbosum ‘Lanmei 1’ were studied. The results show that all rooting indexes of stem segments of tissue culture seedlings of ‘Lanmei 1’ in B2 〔V（moss， M）∶V（porous soil amendments with particle size of 1-3 mm， PM1）=4∶1〕 substrate are the best， in which， rooting rate and comprehensive rooting quality at the 14th day after cutting are significantly higher than those in CK2 （M） substrate. All rooting indexes of stem segments of tissue culture seedlings of ‘Lanmei 1’ in B1 〔V（M）∶V（PM1）=9∶1〕 substrate are also relatively good， and are not significantly different from those in B2 substrate. If considering economic cost， B1 substrate is better.