Liver protective effect of water extract from aboveground part of Passiflora wilsonii Hemsl. （WEPW） on ethanol-induced acute liver injury in mice was evaluated. The results show that compared with normal group， alanine aminotransferase activity and contents of triglyceride， total cholesterol and very low-density lipoprotein in serum， liver index， and contents of triglyceride and malondialdehyde in liver tissue of mice in ethanol-induced acute liver injury group （giving volume fraction of 50% ethanol at dose of 12 mL·kg^-1·d^-1 by gavage） increase significantly or obviously significantly. Compared with ethanol-induced acute liver injury group， alanine aminotransferase activity and triglyceride content in serum， liver index， and contents of triglyceride and malondialdehyde in liver tissue of mice given WEPW at doses of 100， 200， and 400 mg·kg^-1·d^-1 by gavage decrease obviously， and there is a concentration-dependent relationship. WEPW can obviously ameliorate liver accumulation of lipid droplets of mice with ethanol-induced acute liver injury by histopathological section of liver. It is suggested that WEPW has obvious preventive and protective effects on ethanol-induced acute liver injury in 

mice.