摘要 | 研究了不同浓度的NaCl和复合盐及等渗溶液(PEG 6000)处理下盐生植物灰绿藜(Chenopodium glaucum L.)种子的萌发状况。结果表明:灰绿藜种子的萌发率与处理溶液的浓度或渗透势之间有显著的负相关关系;在低浓度盐溶液(2.9g·L-1)中灰绿藜种子的萌发率高于对照(蒸馏水); NaCl溶液对灰绿藜种子萌发的抑制作用大于复合盐溶液。渗透势为-0.2和-0.5mPa时,PEG 6000溶液对灰绿藜种子萌发的抑制作用小于等渗NaCl溶液,而在较高渗透势溶液中则正好相反。用渗透势≤-1.8mPa的PEG 6000溶液及所有浓度的NaCl和复合盐溶液处理的种子复水后相对萌发率都达到了90%以上,说明一定程度的盐分和水分胁迫对灰绿藜种子萌发潜力并没有很大的影响,并且萌发恢复率随处理盐浓度或PEG 6000溶液渗透势(≤-1.4mPa)的增加而增加。 |
Abstract | Seeds of halophyte Chenopodium glaucum L. were treated with various concentration of sodium chloride, mixed salt solutions and PEG-6000 solutions iso-osmotic to sodium chloride solutions. The germination percentage and germination recovery percentage were examined. The results showed that germination percentage decreased with the increase of salinity and osmotic potential and the optimal germination was in salt solution at 2.9 g·L-1.The sodium chloride solutions were more inhibitory than mixed salt solutions to seed germination and were less inhibitory than iso-osmotic PEG-6000 solutions at-0.9,-1.4,-1.8 and-2.7 mPa. When non-germinated seeds from all treatments were transferred to distilled water, the final relative rate of germination was more than 90% except thatfromPEG-6000 at- 2.7 mPa. The results showed that a certain degree of salt stress and water stress were a reversible inhibition to seed germination. The germination recovery percentage increased with an increase of the pre-treated salt concentration and osmotic potential of PEG-6000(≤ - 1.4 mPa). |
关键词 | 灰绿藜; 盐胁迫; 水分胁迫; 萌发率; 萌发恢复率; |
Key words | Chenopodium glaucum L.; salt stress; water stress; germination percentage; germination recovery percentage |
作者 | 段德玉1,2,刘小京1,冯凤莲3,李存桢1,2 |
所在单位 | 1.中国科学院石家庄农业现代化研究所,河北石家庄050021; 2.中国科学院研究生院,北京100039; 3.河北医科大学,河北石家庄050017 |
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基金项目 | 国家科技部中巴政府间合作项目(16 413); 河北省"十五"科技攻关资助项目(03220169D); 中国科学院石家庄农业现代化研究所知识创新方向性项目(220761); |