初步研究了低温条件下枇杷(Eriobotrya japonica Lindl.)试管苗离体保存的效果。结果表明:在常温(25℃)条件下,枇杷试管苗生长速度快,保存时间短,10个月后全部死亡;而在低温条件下,试管苗生长缓慢,保存时间较长。在11℃和15℃条件下,品种’解放钟’和’早钟6号’1a后的死亡率分别为20%、25%和15%、20%,表明低温有利于枇杷种质资源的离体保存。在试管包扎物的选择中,白纸+塑料薄膜和锡箔纸等材料封口对保存有利,而棉花、软木塞、橡皮塞等封口材料不利于试管苗的保存。

The effect of low temperature on the germplasm preservation with the plantlets of Eriobotrya japonica Lindl. was investigated. The results showed that in normal temperature (25℃) the plantlets grew fast, their preservation time was short and all died after 10 month. In low temperature, the plantlets grew slowly, their preservation time was longer than that in normal temperature. At 11℃ and 15℃ , death rates of plantlets of two cultivars `Jiefangzhong' and `Zaozhong No. 6' were only 20%, 25% and 15%, 20% respectively after storage for one year. The low temperature was favorable for germplasm preservation of E. japonica. It was found that both the packingwith the white paper, then covered with polypropylene membrane and the packing with tinfoil paper were suitable for the germplasm preservation of E. japonica, cotton, cork and rubber plug were obviously not suitable as packing materials.