Effect of different planting patterns on total secoiridoid glycoside content in different parts of Gentiana rigescens Franch. ex Hemsl. was researched. The results show that different planting patterns have significant or extremely significant effects on total secoiridoid glycoside content in root, stem and leaf of G. rigescens. Total secoiridoid glycoside content in root is the highest under planting pattern of G. rigescens - Alnus nepalensis D. Don and is the lowest under planting pattern of G. rigescens-Chaenomeles sinensis ( Thouin) Koehne, that in stem is the highest under single planting pattern of G. rigescens and is the lowest under planting pattern of G. rigescens-Juglans regia Linn., and that in leaf is the highest under planting pattern of G. rigescens-Cunninghamia lanceolata ( Lamb.) Hook. and is the lowest under single planting pattern of G. rigescens.