2024年2月25日 星期日
香果树茎段和叶片的组织培养
Tissue culture of stem and leaf of Emmenopterys henryi
2008年 第17卷 第1期 页码[71-74]    下载全文[0.6MB]  
摘要

以香果树(Emmenopterys  henryi Oliv.)实生苗带芽茎段和叶片为外植体进行组织培养。结果表明,香果树带芽茎段愈伤组织的最适诱导培养基为含有1.0mg·L-16-BA和0.01mg·L-1NAA的MS培养基,愈伤组织诱导率达100%;愈伤组织分化的最适培养基为添加2.0mg·L-1KT和0.01mg·L-1NAA的MS培养基;在含有1.0mg·L-16-BA和0.1mg·L-1NAA的MS培养基上,叶片诱导不定芽的效果较好,诱导率可达80%;在含有2.0mg·L-1KT和0.1mg·L-1NAA的MS培养基上,不定芽的增殖系数可达3~4;以含有1.5mg·L-1IBA的1/2MS培养基为生根培养基,香果树试管苗生根率达72.73%。移栽至大田后,香果树试管苗的成活率达到30%。

Abstract

Tissue culture of Emmenopterys henryi Oliv. has been investigated using leaf and stem as explants. The results show that MS medium containing 1.0mg·L- 1 6-BA and 0.01mg·L- 1NAA is suitable for inducing callus from stems, the induced rate reaches to 100%.MSmedium containing 2.0 mg·L- 1KTand 0.01mg·L- 1NAA is the best suitable medium for callus differentiation. On MS medium containing 1.0mg·L- 1 6-BA and 0.1mg·L- 1NAA,inducement effect of adventitious shoot

from leaf is better and the induced rate reaches to 80%.The perfect medium for adventitious shoot proliferation is the MS medium containing 2.0mg·L- 1KTand 0.1mg·L- 1NAA and the proliferation coefficient reaches to 3- 4.Rooting rate of E. henryi plantlets can reach to 72.73%byusing 1/2MS medium containing 1.5mg·L- 1IBA as rooting medium. Survival rate of E. henryi plantlets is about 30% after transplanting to field.

关键词香果树; 组织培养; ;
Key wordsEmmenopterys henryi Oliv.; tissue culture; stem; leaf
作者宿 静1,汤庚国1,万 劲1,汤诗杰1,2,武文婷1,李玉萍1
所在单位1.南京林业大学,江苏南京210037; 2.江苏省•中国科学院植物研究所(南京中山植物园),江苏南京210014
点击量1323
下载次数821
基金项目浙江省教育厅中青年教师资助项目(20020259);