以香果树(Emmenopterys  henryi Oliv.)实生苗带芽茎段和叶片为外植体进行组织培养。结果表明,香果树带芽茎段愈伤组织的最适诱导培养基为含有1.0mg·L^-16-BA和0.01mg·L^-1NAA的MS培养基,愈伤组织诱导率达100%;愈伤组织分化的最适培养基为添加2.0mg·L^-1KT和0.01mg·L^-1NAA的MS培养基;在含有1.0mg·L-16-BA和0.1mg·L-1NAA的MS培养基上,叶片诱导不定芽的效果较好,诱导率可达80%;在含有2.0mg·L^-1KT和0.1mg·L-1NAA的MS培养基上,不定芽的增殖系数可达3~4;以含有1.5mg·L^-1IBA的1/2MS培养基为生根培养基,香果树试管苗生根率达72.73%。移栽至大田后,香果树试管苗的成活率达到30%。

Tissue culture of Emmenopterys henryi Oliv. has been investigated using leaf and stem as explants. The results show that MS medium containing 1.0mg·L^{- 1} 6-BA and 0.01mg·L^{- 1}NAA is suitable for inducing callus from stems, the induced rate reaches to 100%.MSmedium containing 2.0 mg·L^{- 1}KTand 0.01mg·L^{- 1}NAA is the best suitable medium for callus differentiation. On MS medium containing 1.0mg·L^{- 1} 6-BA and 0.1mg·L^{- 1}NAA,inducement effect of adventitious shoot

from leaf is better and the induced rate reaches to 80%.The perfect medium for adventitious shoot proliferation is the MS medium containing 2.0mg·L^{- 1}KTand 0.1mg·L^{- 1}NAA and the proliferation coefficient reaches to 3- 4.Rooting rate of E. henryi plantlets can reach to 72.73%byusing 1/2MS medium containing 1.5mg·L^{- 1}IBA as rooting medium. Survival rate of E. henryi plantlets is about 30% after transplanting to field.