对毛头鬼伞(Coprinus comatus Muell.ex Fr.)子实体中的多糖CCP60a进行了提取分离及检测,研究了不同温度条件下CCP60a对烟草花叶病毒(TMV)外壳蛋白体外聚合的影响,并用Western blotting法研究了CCP60a对TMV外壳蛋白表达的影响。结果表明,经沸水浸提、乙醇分级沉淀、DEAE Sephadex A-25离子交换柱层析、Sepharose-6B凝胶柱层析和抗TMV活性跟踪检测可得到均一的多糖CCP60a。随温度的提高,CCP60a处理组在320nm处的吸光度值增加幅度明显小于对照组,表明CCP60a对TMV外壳蛋白体外聚合有一定的抑制作用。Western blotting检测结果显示,CCP60a可以使TMV外壳蛋白的表达明显降低。 

The polysaccharide CCP60a was extracted and separated from the fruiting bodies of Coprinus comatus Muell.ex Fr. The effect of CCP60a on polymerization of TMV coat protein in vitro was studied under different temperatures, and the effect of CCP60a on expression of TMV coat protein was also tested byWestern blotting method. The results showed that the homogeneous polysaccharide CCP60a was obtained by the boiling water extraction, ethanol precipitation, DEAE SephadexA-25 column chromatography, Sepharose-6B gel column chromatography and anti-TMV activity examination. The increasing extent of absorbance value(320nm) of CCP60 a treatment group was smaller than that of the control within creasing of temperature, this showed that CCP60a had an inhibition effect on polymerization of TMV coat protein in vitro. The result of Western blotting test also indicated that CCP60a could obviously reduced the expression of TMV coat protein.