The chlorogenic acid contents in 13 cultivars of Dendranthema morifolium (Ramat.) Tzvel. from 4 provinces in China were determined by RP-HPLC. The results showed that the contents of chlorogenic acid in different medicinal cultivars of D. morifolium were Hangju ‘Xiao huangju’ 0.243%, Hangju ‘Xiao yangju’0.457%, Hangju ‘Da yangju’ 0.309%, Hangju ‘Yizhong Dayangju’ 0.306%, Hangju ‘Da yangju’ (processed by microwaves) 0.511%,Huaiju ‘Xiao baiju’0.554%, Huaiju ‘Da baiju’0.224%, Huaiju ‘Da huangju’0.655%, Jiju 0.434%, Boju 0.161%, Gongju 0.719%, Chuju 0.397% and Zhonghuagongju 0.590%. The content of chlorogenic acid in Gongju from Huangshan, Anhui Province is higher than those from other cultivars significantly.