2024年7月22日 星期一
生长调节剂对离体银杏叶苯丙氨酸解氨酶活性的影响
Effects of plant growth regulators on phenylalanine ammonia-lyase(PAL) activities in leaves of Ginkgo biloba in vitro
2005年 第14卷 第1期 页码[20-22]    下载全文[0.4MB]  
摘要

用 6种生长调节剂诱导离体银杏 (Ginkgo biloba Linn. )叶苯丙氨酸解氨酶 (PAL)活性, 结果表明: 300mg·L-1 ETP诱导作用最强,其动态诱导在处理 4h后最高; 40mg·L-1 2, 4 D诱导效果最强,并有 2个明显的诱导高峰; 200mg·L-1CCC诱导作用最强,在处理 8h后出现诱导高峰;除 100mg·L-1 IAA处理组的酶活性比对照低外,其他浓度的IAA处理均比对照高,诱导作用最强的是 70mg·L-1 IAA处理;除了 100mg·L-1 ABA处理使酶活性略有降低外,其他浓度的ABA处理都能诱导酶活性升高,以 75mg·L-1 ABA诱导能力最强,在处理 4h后酶活性最大; 4个浓度的GA处理中,仅 75mg·L-1 GA处理组的酶活性高于对照,处理 8h后酶活性达到最大。结果说明诱导效果从高至低依次为:乙烯利 (ETP)、2, 4 D、矮壮素 (CCC)、吲哚乙酸 (IAA)、脱落酸 (ABA)、赤霉素(GA)。

Abstract

The effects of six plant growth regulators on PAL activities in in vitro leaves from Ginkgo biloba Linn. were studied. The results showed that 300mg·L-1 ethephon (ETP)had the strongest effect, and the PAL activities were the highest after4 h. 40 mg·L-12,4-D had the strongest effect with two obvious inducing peaks.200 mg·L-1chlorocholine chloride (CCC) had the strongest effect with one inducing peak after 8 h. The PAL activities of IAA treatments except100 mg·L-1 were higher than that of the contrast(CK), and 70 mg·L-1 IAA had the greatest effect. ABA except 100 mg·L-1 treatment enhanced PAL activities, and 75 mg·L-1 ABA was the optimum treatment. After 75 mg·L-1 ABA treatment4 h, the PAL activities reached zenith. The PAL activitie swith GA treatments were lower than that of CK but 75 mg·L-1 treatment, which presented one inducing peak after 8 h. The inducing influences ranked from high to low are ETP, 2,4-D, CCC, IAA, ABA, GA.

关键词生长调节剂; 叶片; 银杏; 苯丙氨酸解氨酶活性;
Key wordsplant growth regulator; leaf; Ginkgo biloba Linn.; PAL activity
作者程水源1,2,王燕1,刘卫红1,杜何为1,陈昆松2
所在单位1.长江大学园艺园林学院,湖北荆州434025;
2.浙江大学果树研究所,浙江杭州310000
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基金项目中国教育部科技攻关计划(02095); 湖北省科技重点攻关计划(991P1004); 湖北省自然科学基金 ( 2002AB094 ); 湖北省青年杰出人才基金(2003AB014)资助项目;